Sex-related difference in oxidative metabolism of testosterone and erythromycin by hamster liver microsomes

FEBS Lett. 1988 Apr 11;231(1):183-6. doi: 10.1016/0014-5793(88)80727-0.


The activities of testosterone hydroxylases and erythromycin N-demethylase were significantly higher in liver microsomes from female hamsters than in the male counterparts. SDS-polyacrylamide gel electrophoresis revealed a difference in protein composition between male and female liver microsomes in the molecular mass region comprising cytochrome P-450. Western blot analysis showed further that antibodies to rat male-specific cytochrome P-450 crossreacted with at least two proteins in both male and female hamster microsomes, but one of the female proteins had a different molecular mass from that of the male proteins. It is concluded that sex difference in liver microsomal cytochrome P-450 is not restricted to rats and mice, as has previously been believed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aryl Hydrocarbon Hydroxylases*
  • Cricetinae
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 Enzyme System / isolation & purification
  • Cytochrome P-450 Enzyme System / metabolism
  • Cytochrome b Group / isolation & purification
  • Cytochrome b Group / metabolism
  • Cytochromes b5
  • Erythromycin / metabolism*
  • Female
  • Male
  • Mesocricetus
  • Microsomes, Liver / enzymology*
  • Oxidation-Reduction
  • Oxidoreductases, N-Demethylating / metabolism*
  • Sex Factors
  • Steroid Hydroxylases / metabolism*
  • Testosterone / metabolism*


  • Cytochrome b Group
  • Testosterone
  • Erythromycin
  • Cytochromes b5
  • Cytochrome P-450 Enzyme System
  • Steroid Hydroxylases
  • Aryl Hydrocarbon Hydroxylases
  • Cytochrome P-450 CYP3A
  • testosterone 7-alpha-hydroxylase, hamster
  • Oxidoreductases, N-Demethylating