Transposon-Associated CRISPR-Cas System: A Powerful DNA Insertion Tool

Wang Ma; Ying-Shuang Xu; Xiao-Man Sun; He Huang

doi:10.1016/j.tim.2021.01.017

Transposon-Associated CRISPR-Cas System: A Powerful DNA Insertion Tool

Trends Microbiol. 2021 Jul;29(7):565-568. doi: 10.1016/j.tim.2021.01.017. Epub 2021 Feb 18.

Authors

Wang Ma¹, Ying-Shuang Xu¹, Xiao-Man Sun², He Huang³

Affiliations

¹ School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing, People's Republic of China.
² School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing, People's Republic of China. Electronic address: xiaomansun@njnu.edu.cn.
³ School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 2 Xuelin Road, Qixia District, Nanjing, People's Republic of China; College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, People's Republic of China. Electronic address: huangh@njnu.edu.cn.

PMID: 33612399
DOI: 10.1016/j.tim.2021.01.017

Abstract

The transposon-associated CRISPR-Cas system is emerging as a powerful tool for targeted genetic manipulation. Using this system, targeted genomic knockins and knockouts can be achieved, independently of the host-cell repair machinery, thereby significantly improving our ability to perform genome editing in a wider variety of cell types.

Keywords: DNA insertion; microbial community genome editing; targeted genome editing; transposable elements; transposon-associated CRISPR-Cas system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems*
DNA Transposable Elements*
Gene Editing / methods*
Genome*
Humans

Substances

DNA Transposable Elements