Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release

STAR Protoc. 2021 Feb 8;2(1):100333. doi: 10.1016/j.xpro.2021.100333. eCollection 2021 Mar 19.


In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as a possible downstream application. In addition, isolated DRGs can be used directly for immunofluorescence, flow cytometry, RNA sequencing or proteomic approaches, electrophysiology, and calcium imaging. For complete details on the use and execution of this protocol, please refer to Perner et al. (2020).

Keywords: Cell culture; Cell isolation; Cell-based assays; Neuroscience.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / metabolism*
  • Mice
  • Neuropeptides / metabolism*
  • Proteomics*
  • Sensory Receptor Cells / cytology
  • Sensory Receptor Cells / metabolism*
  • Tissue Culture Techniques


  • Neuropeptides