Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates Against Bacterial Strains

Ume Habiba; Jaweria Nisar; Muhammad Akram Choohan; Syed Muhammad Ali Shah; Zonaira Nisar; Imtiaz Mustafa

doi:10.1177/1559325821992239

Antibacterial Activity of Tris NaCl and PBS Buffer Protein Extract of Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates Against Bacterial Strains

Dose Response. 2021 Feb 11;19(1):1559325821992239. doi: 10.1177/1559325821992239. eCollection 2021 Jan-Mar.

Authors

Ume Habiba^{1

2}, Jaweria Nisar³, Muhammad Akram Choohan², Syed Muhammad Ali Shah³, Zonaira Nisar³, Imtiaz Mustafa⁴

Affiliations

¹ Department of Microbiology and Molecular genetics, The Women University Multan, Multan, Punjab, Pakistan.
² University College of Conventional Medicine, Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan.
³ Department of Eastern Medicine, Government College University Faisalabad, Faisalabad, Punjab, Pakistan.
⁴ Department of Physiology, Government College University Faisalabad, Faisalabad, Punjab, Pakistan.

Abstract

Medicinal plants are gaining popularity over synthetic medicines because antibiotic resistance demands the alternative source of medication. In the present research, the crude protein extraction of 4 medicinal plants Cassia fistula, Saccharum officinarum, Albizia lebbeck and Cymbopogon citrates was carried out. Crude protein extraction was done by 2 different buffers i.e. Tris NaCl buffer and PBS buffer. Protein confirmation was done by Bradford assay in the spectrophotometer. Antibacterial potential was checked and compared against Escherichia coli, Bacillus subtilis, Neisseria gonorrhoea, Bacillus cereus and Proteus mirabilis. Antibacterial assay was performed by disc diffusion method, agar well method and zones of inhibition were calculated. The study results indicated that Tris NaCl extracts' antimicrobial potential is higher than that of the PBS buffer. On disc diffusion method the Tris NaCl buffer extracts of Cymbopogon citrates showed maximum zone of inhibition 11 mm and 9 mm against Bacillus subtilis and Bacillus cereus respectively and control chloramphenicol showed maximum zone of inhibition 26 mm against Bacillus subtilis. Cassia fistula showed maximum zone of inhibition of 7 mm against Bacillus cereus while Saccharum officinarum and Albizia lebbeck didn't show the any antibacterial activity. On the other hand, Protein extracts from PBS buffer didn't show zone of inhibition against any bacteria. Only Albizia lebbeck showed minute zone of inhibition against Neisseria gonorrhea. On well diffusion method, Cassia fistula Tris NaCl protein extract showed the maximum zone of inhibition 20 mm and 18 mm against Proteus mirabilis and Bacillus subtilis respectively. While Albizia lebbeck PBS protein extract showed the maximum zone of inhibition 19 mm and 17 mm against Bacillus subtilis and Bacillus cereus. The results revealed that the protein extract of Albizia lebbeck, Cymbopogon citrates and Cassia fistula can be used tosynthesize antimicrobial drugs to treat the bacterial infections.

Keywords: PBS buffer; antibacterial activity; bacillus cereus; bacillus subtilis; cassia fistula; cymbopogon citrates; protein extract; tris NaCl.