Qingshengfaniella alkalisoli gen. nov., sp. nov., a p-hydroxybenzoate-degrading strain isolated from saline soil

Shuai Wang; Rui Yang; Lian Xu; Ya-Ting Xing; Ji-Quan Sun

doi:10.1099/ijsem.0.004719

Qingshengfaniella alkalisoli gen. nov., sp. nov., a p-hydroxybenzoate-degrading strain isolated from saline soil

Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004719. Epub 2021 Feb 25.

Authors

Shuai Wang¹, Rui Yang¹, Lian Xu¹, Ya-Ting Xing¹, Ji-Quan Sun¹

Affiliation

¹ Department of Environmental Engineering, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, PR China.

PMID: 33629940
DOI: 10.1099/ijsem.0.004719

Abstract

p-Hydroxybenzoate is an allelopathic compound commonly found in soil from long-term monoculture cropping systems. During the bacterial diversity analysis of saline soil, a Gram-negative, non-spore forming, non-motile, aerobic p-hydroxybenzoate-degrading bacterial strain, designated LN3S51^T, was isolated from saline soil which was sampled from Tumd Right Banner, Inner Mongolia, northern China. Strain LN3S51^T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0) and 0-15 % NaCl (optimum 3.0 %). Though strain LN3S51^T has the highest 16S rRNA gene similarities to Litoreibacter ponti GJSW-31^T (96.0 %), the phylogenetic tree based on the 16S rRNA gene sequences showed that it clustered with Fluviibacterium aquatile SM1902^T (95.8 %), Meridianimarinicoccus roseus TG-679^T (93.9 %), and Phycocomes zhengii LMIT002^T (93.9 %). Strain LN3S51^T contained Q-10 as the major ubiquinone. Phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), diphosphatidylglycerol (DPG), an unidentified aminolipid (AL), and two unidentified lipids (L) were the major polar lipids. The major fatty acids were sum feature 8 (C_{18 : 1} ω7c and/or C_{18 : 1} ω6c), C_{16 : 0}, C_{18 : 0}, and C_{18 : 1} ω7c 11-methyl. The genome of strain LN3S51^T consisted of a 2 257 066 bp chromosome and four plasmids with a 59.1 mol% of genomic DNA G+C content. The average nucleotide identity (ANI) and digital DNA-DNA hybridization score (dDDH) values of strain LN3S51^T to F. aquatile SM1902^T, M. roseus TG-679^T, P. zhengii LMIT002^T, and L. ponti GJSW-31^T were 69.6, 70.9, 70.6, and 69.5 %, and 20.0, 19.5, 19.0, and 20.0 %, respectively. Based on the results of phylogenetic, chemtaxonomic and phenotypic characterization, strain LN3S51^T is considered to represent a novel species in a new genus within the family Rhodobacteraceae, for which Qingshengfaniella alkalisoli gen. nov., sp. nov. is proposed. The type strain is LN3S51^T (=CGMCC 1.17099^T=KCTC 72457^T).

Keywords: Alphaproteobacteria; Biodegradation saline soil; Hydroxybenzoate; Taxonomy; phylogenetic analysis.