Abstract
Eosin Y was assessed for its ability to induce a thiol-ene dependent protein-protein reaction in a metal-free, oxygen-tolerant, visible light mediated system. Protein-protein coupling efficiency under these mild conditions was comparable to previously reported UV-dependent conditions. The desired thiol-ene reaction was however limited within more complex biological systems.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkenes / chemistry
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Catalysis / radiation effects
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Cysteine / chemistry
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Cysteine Endopeptidases / chemistry*
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Deubiquitinating Enzymes / chemistry*
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Eosine Yellowish-(YS) / chemistry*
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Eosine Yellowish-(YS) / radiation effects
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HEK293 Cells
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Humans
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Light
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Molecular Probes / chemistry*
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Molecular Probes / radiation effects
Substances
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Alkenes
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Molecular Probes
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Deubiquitinating Enzymes
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OTUB1 protein, human
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Cysteine Endopeptidases
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Cysteine
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Eosine Yellowish-(YS)