Investigating eosin Y as a photocatalyst for the radical-dependent activity-based probing of deubiquitinating enzymes

Org Biomol Chem. 2021 Mar 18;19(10):2177-2181. doi: 10.1039/d1ob00253h.

Abstract

Eosin Y was assessed for its ability to induce a thiol-ene dependent protein-protein reaction in a metal-free, oxygen-tolerant, visible light mediated system. Protein-protein coupling efficiency under these mild conditions was comparable to previously reported UV-dependent conditions. The desired thiol-ene reaction was however limited within more complex biological systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkenes / chemistry
  • Catalysis / radiation effects
  • Cysteine / chemistry
  • Cysteine Endopeptidases / chemistry*
  • Deubiquitinating Enzymes / chemistry*
  • Eosine Yellowish-(YS) / chemistry*
  • Eosine Yellowish-(YS) / radiation effects
  • HEK293 Cells
  • Humans
  • Light
  • Molecular Probes / chemistry*
  • Molecular Probes / radiation effects

Substances

  • Alkenes
  • Molecular Probes
  • Deubiquitinating Enzymes
  • OTUB1 protein, human
  • Cysteine Endopeptidases
  • Cysteine
  • Eosine Yellowish-(YS)