Peribacillus faecalis sp. nov., a moderately halophilic bacterium isolated from the faeces of a cow

Abstract

A Gram-stain-positive, facultatively anaerobic, endospore-forming, rod-shaped strain, AGMB 02131^T, which grew at 20-40 °C (optimum 30 °C), pH 3.0-11.0 (optimum pH 4.0) and in the presence of 0-18 % (w/v) NaCl (optimum 10 %), was isolated from a cow faecal sample and identified as a novel strain using a polyphasic taxonomic approach. The phylogenetic analysis based on 16S rRNA gene sequences along with the whole genome (92 core gene sets) revealed that AGMB 02131^T formed a group within the genus Peribacillus, and showed the highest sequence similarity with Peribacillus endoradicis DSM 28131^T (96.9 %), following by Peribacillus butanolivorans DSM 18926^T (96.6 %). The genome of AGMB 02131^T comprised 70 contigs, the chromosome length was 4 038 965 bp and it had a 38.5 % DNA G+C content. Digital DNA-DNA hybridization revealed that AGMB 02131^T displayed 21.4 % genomic DNA relatedness with the most closely related strain, P. butanolivorans DSM 18926^T. AGMB 02131^T contains all of the conserved signature indels that are specific for members of the genus Peribacillus. The major cellular fatty acids (>10 %) of AGMB 02131^T were C_{18 : 1}ω9c, C_18:0 and C_{16 : 0}. The major polar lipids present were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the phenotypic, phylogenetic, genomic and chemotaxonomic features, AGMB 02131^T represents a novel species of the genus Peribacillus, for which the name Peribacillus faecalis sp. nov. is proposed. The type strain is AGMB 02131^T (=KCTC 43221^T=CCTCC AB 2020077^T).

Keywords: Peribacillus faecalis sp. nov; novel species; phylogeny; polyphasic taxonomy.