Urinary Exosomes in Prostate Cancer

Methods Mol Biol. 2021;2292:115-120. doi: 10.1007/978-1-0716-1354-2_10.

Abstract

The analysis of liquid biopsy as a source of diagnostic, prognostic, and predictive biomarkers is still object of the main research in the prostate cancer field. Many advantages, such as less invasiveness compared to plasma or serum analysis and the rich content, confer to urine a role as an interesting fluid to be analysed especially in urological diseases. Here we report a workflow focused on profile, concentration, and protein surface characterization of EVs from urinary supernatant.

Keywords: MACSPlex kit; Prostate cancer; Urinary exosomes; Urinary supernatant.

MeSH terms

  • Biomarkers, Tumor / analysis
  • Biomarkers, Tumor / urine
  • Exosomes / pathology*
  • Humans
  • Liquid Biopsy / methods
  • Male
  • Prostatic Neoplasms / diagnosis*
  • Prostatic Neoplasms / pathology
  • Prostatic Neoplasms / urine
  • Proteins / analysis
  • Proteinuria / diagnosis
  • Proteinuria / pathology
  • Proteinuria / urine
  • Urine Specimen Collection / methods
  • Workflow

Substances

  • Biomarkers, Tumor
  • Proteins