Two-photon microperimetry with picosecond pulses

Marcin J Marzejon; Łukasz Kornaszewski; Jakub Bogusławski; Piotr Ciąćka; Miłosz Martynow; Grażyna Palczewska; Sebastian Maćkowski; Krzysztof Palczewski; Maciej Wojtkowski; Katarzyna Komar

doi:10.1364/BOE.411168

Two-photon microperimetry with picosecond pulses

Biomed Opt Express. 2020 Dec 17;12(1):462-479. doi: 10.1364/BOE.411168. eCollection 2021 Jan 1.

Authors

Marcin J Marzejon^{1

2

3}, Łukasz Kornaszewski^{1

3}, Jakub Bogusławski^{1

3}, Piotr Ciąćka^{1

3}, Miłosz Martynow^{4

5}, Grażyna Palczewska^{3

6

7}, Sebastian Maćkowski^{4

8}, Krzysztof Palczewski^{3

7

9

10}, Maciej Wojtkowski^{1

3

4

8}, Katarzyna Komar^{3

4

8}

Affiliations

¹ Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warszawa, Poland.
² Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, G. Narutowicza 11/12, 80-223 Gdańsk, Poland.
³ International Centre for Translational Eye Research, Institute of Physical Chemistry, PAS, Skierniewicka 10a, 01-230 Warszawa, Poland.
⁴ Baltic Institute of Technology, Al. Zwycięstwa 96/98, 81-451 Gdynia, Poland.
⁵ Department of Theoretical Physics and Quantum Information, Faculty of Applied Physics and Mathematics, Gdańsk University of Technology, G. Narutowicza 11/12, 80-233 Gdańsk, Poland.
⁶ Polgenix, Inc., Department of Medical Devices, Cleveland OH 44106, USA.
⁷ Gavin Herbert Eye Institute, Department of Ophthalmology, University of California, Irvine, CA 92697, USA.
⁸ Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Torun, Grudziądzka 5, 87-100 Toruń, Poland.
⁹ Department of Physiology & Biophysics, School of Medicine, University of California, Irvine, CA 926978, USA.
¹⁰ Department of Chemistry, University of California, Irvine, CA 926978, USA.

Abstract

Two-photon vision is a phenomenon associated with the perception of short pulses of near-infrared radiation (900-1200 nm) as a visible light. It is caused by the nonlinear process of two-photon absorption by visual pigments. Here we present results showing the influence of pulse duration and repetition rate of short pulsed lasers on the visual threshold. We compared two-photon sensitivity maps of the retina obtained for subjects with normal vision using a cost-effective fiber laser (λ_c = 1028.4 nm, τ_p = 12.2 ps, F_rep = 19.17 MHz) and a solid-state laser (λ_c = 1043.3 nm, τ_p = 0.253 ps, F_rep = 62.65 MHz). We have shown that in accordance with the description of two-photon absorption, the average optical power required for two-photon vision for a fiber laser is 4 times greater than that for a solid-state laser. Mean sensitivity measured for the first one is 5.9 ± 2.8 dB lower than for the second but still 17 dB away from the safety limit, confirming that picosecond light sources can be successfully applied in microperimetry. This development would dramatically reduce the cost and complexity of future clinical devices.

Grants and funding

U01 EY025451/EY/NEI NIH HHS/United States