Organotypic Slice Culture of the Embryonic Mouse Brain

Abstract

Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.

Keywords: Axon guidance; Bead culture; Morphogen; Organotypic; Slice culture; Transplant.