The Notch signaling pathway is an important conserved pathway for normal homeostasis during development. However, targeted deletion of Notch4 (Notch4d1 ) or Notch3 (Notch3d1 ) in mice is not lethal. In fact, both Notch4d1 and Notch3d1 mice develop normally and are fertile. Here we present RNA seq analysis of differential gene expression in the kidneys of Notch4d1 mice versus the Notch3 d1 mice, all on FVB background. Kidneys were collected from Notch4d1 and Notch3 d1 littermates at 3 months of age. RNA sequencing was carried out. The raw data were analyzed for differential gene expression using a negative binomial generalized linear model in the DeSeq2 software package. We used P-value ≤0.05 and an absolute fold change of 1.5 or greater to identify top upregulated and downregulated genes in Notch4 d1 mice compared to Notch3 d1 mice. The data provided will indentify targets of Notch3 and Notch4 signaling, specifically in kidney diseases where Notch3 or Notch4 are abberantly or redundantly expressed.
Keywords: Chronic kidney disease; HIVAN; Inflammation; Tg26.
© 2021 The Authors. Published by Elsevier Inc.