Updating the influenza virus library at Hokkaido University -It's potential for the use of pandemic vaccine strain candidates and diagnosis

Virology. 2021 May;557:55-61. doi: 10.1016/j.virol.2021.02.005. Epub 2021 Feb 22.


Genetic reassortment of influenza A viruses through cross-species transmission contributes to the generation of pandemic influenza viruses. To provide information on the ecology of influenza viruses, we have been conducting a global surveillance of zoonotic influenza and establishing an influenza virus library. Of 4580 influenza virus strains in the library, 3891 have been isolated from over 70 different bird species. The remaining 689 strains were isolated from humans, pigs, horses, seal, whale, and the environment. Phylogenetic analyses of the HA genes of the library isolates demonstrate that the library strains are distributed to all major known clusters of the H1, H2 and H3 subtypes of HA genes that are prevalent in humans. Since past pandemic influenza viruses are most likely genetic reassortants of zoonotic and seasonal influenza viruses, a vast collection of influenza A virus strains from various hosts should be useful for vaccine preparation and diagnosis for future pandemics.

Keywords: Hemagglutinin; Influenza virus library; Pandemic influenza vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fur Seals / virology
  • Gene Library*
  • Horses / virology
  • Humans
  • Influenza Vaccines / immunology*
  • Influenza, Human / diagnosis*
  • Influenza, Human / virology
  • Orthomyxoviridae / genetics*
  • Orthomyxoviridae / isolation & purification
  • Orthomyxoviridae Infections / veterinary*
  • Orthomyxoviridae Infections / virology*
  • Pandemics / prevention & control*
  • Phylogeny
  • Reassortant Viruses
  • Swine / virology
  • Universities*


  • Influenza Vaccines