A Novel Protein from Ectocarpus sp. Improves Salinity and High Temperature Stress Tolerance in Arabidopsis thaliana

Int J Mol Sci. 2021 Feb 17;22(4):1971. doi: 10.3390/ijms22041971.

Abstract

Brown alga Ectocarpus sp. belongs to Phaeophyceae, a class of macroalgae that evolved complex multicellularity. Ectocarpus sp. is a dominant seaweed in temperate regions, abundant mostly in the intertidal zones, an environment with high levels of abiotic stresses. Previous transcriptomic analysis of Ectocarpus sp. revealed several genes consistently induced by various abiotic stresses; one of these genes is Esi0017_0056, which encodes a protein with unknown function. Bioinformatics analyses indicated that the protein encoded by Esi0017_0056 is soluble and monomeric. The protein was successfully expressed in Escherichia coli,Arabidopsis thaliana and Nicotiana benthamiana. In A. thaliana the gene was expressed under constitutive and stress inducible promoters which led to improved tolerance to high salinity and temperature stresses. The expression of several key abiotic stress-related genes was studied in transgenic and wild type A. thaliana by qPCR. Expression analysis revealed that genes involved in ABA-induced abiotic stress tolerance, K+ homeostasis, and chaperon activities were significantly up-regulated in the transgenic line. This study is the first report in which an unknown function Ectocarpus sp. gene, highly responsive to abiotic stresses, was successfully expressed in A. thaliana, leading to improved tolerance to salt and temperature stress.

Keywords: Arabidopsis thaliana; Ectocarpus sp.; abiotic stress tolerance; salinity; temperature; transgenic plant; unknown function protein.

MeSH terms

  • Adaptation, Physiological* / genetics
  • Algal Proteins / chemistry
  • Algal Proteins / genetics
  • Algal Proteins / metabolism*
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / physiology*
  • Electrolytes / metabolism
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Plant
  • Hot Temperature*
  • Phaeophyta / metabolism*
  • Phylogeny
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics
  • Salinity*
  • Seedlings / genetics
  • Stress, Physiological* / genetics
  • Tobacco / metabolism

Substances

  • Algal Proteins
  • Electrolytes