New Insights into the Transcriptional Regulation of Genes Involved in the Nitrogen Use Efficiency under Potassium Chlorate in Rice ( Oryza sativa L.)

Int J Mol Sci. 2021 Feb 22;22(4):2192. doi: 10.3390/ijms22042192.

Abstract

Potassium chlorate (KClO3) has been widely used to evaluate the divergence in nitrogen use efficiency (NUE) between indica and japonica rice subspecies. This study investigated the transcriptional regulation of major genes involved in the NUE in rice treated with KClO3, which acts as an inhibitor of the reducing activity of nitrate reductase (NR) in higher plants. A set of two KClO3 sensitive nitrate reductase (NR) and two nitrate transporter (NRT) introgression rice lines (BC2F7), carrying the indica alleles of NR or NRT, derived from a cross between Saeilmi (japonica, P1) and Milyang23 (indica, P2), were exposed to KClO3 at the seedling stage. The phenotypic responses were recorded 7 days after treatment, and samples for gene expression, physiological, and biochemical analyses were collected at 0 h (control) and 3 h after KClO3 application. The results revealed that Saeilmi (P1, japonica) and Milyang23 (P2, indica) showed distinctive phenotypic responses. In addition, the expression of OsNR2 was differentially regulated between the roots, stem, and leaf tissues, and between introgression lines. When expressed in the roots, OsNR2 was downregulated in all introgression lines. However, in the stem and leaves, OsNR2 was upregulated in the NR introgression lines, but downregulation in the NRT introgression lines. In the same way, the expression patterns of OsNIA1 and OsNIA2 in the roots, stem, and leaves indicated a differential transcriptional regulation by KClO3, with OsNIA2 prevailing over OsNIA1 in the roots. Under the same conditions, the activity of NR was inhibited in the roots and differentially regulated in the stem and leaf tissues. Furthermore, the transcriptional divergence of OsAMT1.3 and OsAMT2.3, OsGLU1 and OsGLU2, between NR and NRT, coupled with the NR activity pattern in the roots, would indicate the prevalence of nitrate (NO3¯) transport over ammonium (NH4+) transport. Moreover, the induction of catalase (CAT) and polyphenol oxidase (PPO) enzyme activities in Saeilmi (P1, KClO3 resistant), and the decrease in Milyang23 (P2, KClO3 sensitive), coupled with the malondialdehyde (MDA) content, indicated the extent of the oxidative stress, and the induction of the adaptive response mechanism, tending to maintain a balanced reduction-oxidation state in response to KClO3. The changes in the chloroplast pigments and proline content propose these compounds as emerging biomarkers for assessing the overall plant health status. These results suggest that the inhibitory potential of KClO3 on the reduction activity of the nitrate reductase (NR), as well as that of the genes encoding the nitrate and ammonium transporters, and glutamate synthase are tissue-specific, which may differentially affect the transport and assimilation of nitrate or ammonium in rice.

Keywords: glutamate synthase; nitrate reductase; nitrate transporter; nitrogen use efficiency; potassium chlorate; rice; transcriptional regulation.

MeSH terms

  • Carotenoids / metabolism
  • Chlorates / pharmacology*
  • Chlorophyll / metabolism
  • Gene Expression Regulation, Plant / drug effects
  • Glutamate Synthase / genetics
  • Glutamate Synthase / metabolism
  • Lipid Peroxidation / drug effects
  • Nitrate Reductase / genetics
  • Nitrate Reductase / metabolism
  • Nitrogen / metabolism*
  • Oryza / drug effects*
  • Oryza / genetics*
  • Oryza / metabolism
  • Phenotype
  • Plant Leaves / metabolism
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Roots / metabolism
  • Proline / metabolism
  • Seedlings / drug effects
  • Seedlings / genetics
  • Seedlings / metabolism

Substances

  • Chlorates
  • Plant Proteins
  • Chlorophyll
  • Carotenoids
  • Proline
  • Glutamate Synthase
  • Nitrate Reductase
  • Nitrogen
  • chloric acid