Characterization of encapsulated porcine cardiosphere-derived cells embedded in 3D alginate matrices

Int J Pharm. 2021 Apr 15;599:120454. doi: 10.1016/j.ijpharm.2021.120454. Epub 2021 Mar 5.

Abstract

Myocardial infarction is caused by an interruption of coronary blood flow, leading to one of the main death causes worldwide. Current therapeutic approaches are palliative and not able to solve the loss of cardiac tissue. Cardiosphere derived cells (CDCs) reduce scarring, and increase viable myocardium, with safety and adequate biodistribution, but show a low rate engraftment and survival after implantation. In order to solve the low retention, we propose the encapsulation of CDCs within three-dimensional alginate-poly-L-lysine-alginate matrix as therapy for cardiac regeneration. In this work, we demonstrate the encapsulation of CDCs in alginate matrix, with no decrease in viability over a month, and showing the preservation of CDCs phenotype, differentiation potential, gene expression profile and growth factor release after encapsulation, moving a step forward to clinical translation of CDCs therapy in regeneration in heart failure.

Keywords: Alginate; Cardiosphere derived cells; Cell microencapsulation; Mesenchymal stem cells; Myocardial infraction.

MeSH terms

  • Alginates
  • Animals
  • Cell Differentiation
  • Heart
  • Myocardium*
  • Myocytes, Cardiac
  • Stem Cell Transplantation*
  • Swine
  • Tissue Distribution

Substances

  • Alginates