Detection of Non-Small Lung Cell Carcinoma-Associated Genetic Alterations Using a NanoString Gene Expression Platform Approach

Methods Mol Biol. 2021;2279:91-107. doi: 10.1007/978-1-0716-1278-1_8.

Abstract

In non-small cell lung cancer (NSCLC), mutation detection and fusion gene status are treatment predictive and, hence, key factors in clinical management. Lately, alternate splicing variants of MET have gained focus as NSCLC tumors harboring a MET exon 14 skipping event have proven sensitive toward targeted therapy. Reliable methods for detection of genetic alterations in NSCLC have proven to be of increased importance. This chapter provides with hands-on experience of the NanoString gene expression platform for detection of genetic alterations in NSCLC.

Keywords: Fusion gene; Gene expression; NanoString; Non-small cell lung carcinoma.

MeSH terms

  • Alternative Splicing*
  • Carcinoma, Non-Small-Cell Lung* / diagnosis
  • Carcinoma, Non-Small-Cell Lung* / enzymology
  • Carcinoma, Non-Small-Cell Lung* / genetics
  • Exons*
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Lung Neoplasms* / diagnosis
  • Lung Neoplasms* / enzymology
  • Lung Neoplasms* / genetics
  • Proto-Oncogene Proteins c-met* / biosynthesis
  • Proto-Oncogene Proteins c-met* / genetics

Substances

  • MET protein, human
  • Proto-Oncogene Proteins c-met