Shear and Integrin Outside-In Signaling Activate NADPH-Oxidase 2 to Promote Platelet Activation

Arterioscler Thromb Vasc Biol. 2021 May 5;41(5):1638-1653. doi: 10.1161/ATVBAHA.120.315773. Epub 2021 Mar 11.

Abstract

[Figure: see text].

Keywords: glycoprotein; integrin; platelet activation; reactive oxygen species; thrombosis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Platelets / enzymology*
  • Enzyme Activation
  • Female
  • GTP-Binding Protein alpha Subunits, G12-G13 / genetics
  • GTP-Binding Protein alpha Subunits, G12-G13 / metabolism
  • Hydrogen Peroxide / metabolism*
  • Integrin beta3 / genetics
  • Integrin beta3 / metabolism*
  • Male
  • Mechanotransduction, Cellular*
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NADPH Oxidase 2 / genetics
  • NADPH Oxidase 2 / metabolism*
  • NADPH Oxidases / metabolism
  • Phosphatidylinositol 3-Kinase / metabolism
  • Phosphorylation
  • Platelet Activation*
  • Platelet Factor 4 / genetics
  • Platelet Factor 4 / metabolism
  • Platelet Glycoprotein GPIIb-IIIa Complex / genetics
  • Platelet Glycoprotein GPIIb-IIIa Complex / metabolism*
  • Proto-Oncogene Proteins c-akt / metabolism
  • Stress, Mechanical
  • Syk Kinase / metabolism

Substances

  • Integrin beta3
  • Platelet Glycoprotein GPIIb-IIIa Complex
  • Platelet Factor 4
  • Hydrogen Peroxide
  • Cybb protein, mouse
  • NADPH Oxidase 2
  • NADPH Oxidases
  • neutrophil cytosolic factor 1
  • Phosphatidylinositol 3-Kinase
  • Syk Kinase
  • Syk protein, mouse
  • Proto-Oncogene Proteins c-akt
  • GTP-Binding Protein alpha Subunits, G12-G13