Detection of Melanoma Cells in Lymphatic Drainage (LD) After Lymph Nodes Dissection Via Nested RT-PCR Analysis of Molecular Melanocytic Markers

Aleksandra Gos; Piotr Rutkowski; Janusz A Siedlecki

doi:10.1007/978-1-0716-1205-7_27

Detection of Melanoma Cells in Lymphatic Drainage (LD) After Lymph Nodes Dissection Via Nested RT-PCR Analysis of Molecular Melanocytic Markers

Methods Mol Biol. 2021:2265:377-384. doi: 10.1007/978-1-0716-1205-7_27.

Authors

Aleksandra Gos¹, Piotr Rutkowski², Janusz A Siedlecki¹

Affiliations

¹ Department of Molecular and Translational Oncology, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.
² Department of Soft Tissue/Bone Sarcoma and Melanoma, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland. piotr.rutkowski@pib-nio.pl.

PMID: 33704728
DOI: 10.1007/978-1-0716-1205-7_27

Abstract

We present the assay based on multimarker analysis of mRNA transcripts associated with melanocytic cells detected in lymphatic fluid collected after lymph node dissection. Positive results of reverse transcriptase polymerase chain reaction (RT-PCR) test have a strong relationship with melanoma recurrence and disease-specific survival time in stage III melanoma.

Keywords: Lymph node metastases; Lymphatic drainage; Melanoma; RT-PCR.

MeSH terms

Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Humans
Lymph Nodes / metabolism*
Lymph Nodes / pathology
Lymphatic Metastasis
Melanocytes / metabolism*
Melanocytes / pathology
Melanoma / genetics
Melanoma / metabolism*
Melanoma / pathology
Neoplasm Recurrence, Local
Neoplasm Staging
Reverse Transcriptase Polymerase Chain Reaction*

Substances

Biomarkers, Tumor