Efficient transformation of mammalian cells with constructs containing a puromycin-resistance marker

Gene. 1988;62(1):121-6. doi: 10.1016/0378-1119(88)90585-9.

Abstract

Recombinant plasmids have been obtained that lead to the accumulation of five- to ten-fold more puromycin-N-acetyl-transferase (PAC) mRNA and two- to three-fold more PAC activity than the already described plasmid pSV2pac [Vara et al., Nucl. Acids Res. 14 (1986) 4117-4124]. When these optimized recombinants were used for stable transformation to puromycin resistance, efficiencies up to 1 x 10(-2) were obtained, indicating that these pac-containing recombinants may be very useful dominant selectable markers for gene transfer in mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / biosynthesis
  • Acetyltransferases / genetics*
  • Animals
  • Cell Line
  • Cricetinae
  • Drug Resistance
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Genetic Vectors*
  • Kidney
  • L Cells / drug effects
  • L Cells / metabolism
  • Mesocricetus
  • Mice
  • Plasmids
  • Puromycin / pharmacology
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Transformation, Genetic*
  • Vero Cells / drug effects
  • Vero Cells / metabolism

Substances

  • Recombinant Fusion Proteins
  • Puromycin
  • Acetyltransferases
  • puromycin N-acetyltransferase