In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation

STAR Protoc. 2021 Mar 2;2(1):100367. doi: 10.1016/j.xpro.2021.100367. eCollection 2021 Mar 19.

Abstract

Little is known about the emergence of blood progenitors during human embryogenesis due to ethical reasons and restricted embryo access. The use of human embryonic stem cells (hESCs) as a model system offers unique opportunities to dissect human blood cell formation. Here, we describe a protocol allowing the differentiation of hESCs via embryoid bodies toward hemogenic endothelium and its subsequent differentiation to blood progenitors. This protocol relies on the formation of embryoid bodies, which is tricky if not carefully performed. For complete details on the use and execution of this protocol, please refer to Garcia-Alegria et al. (2018).

Keywords: Cell culture; Cell differentiation; Cell isolation; Flow cytometry/mass cytometry; Stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / drug effects*
  • Embryoid Bodies / cytology*
  • Embryonic Stem Cells / cytology
  • Flow Cytometry
  • Hemangioblasts
  • Hematopoiesis
  • Hematopoietic Stem Cells / cytology*
  • Human Embryonic Stem Cells / cytology
  • Humans
  • Pluripotent Stem Cells / cytology