Metal Complex Lipids for Fluid-Fluid Phase Separation in Coassembled Phospholipid Membranes

Ryo Ohtani; Yuka Anegawa; Hikaru Watanabe; Yutaro Tajima; Masanao Kinoshita; Nobuaki Matsumori; Kenichi Kawano; Saeko Yanaka; Koichi Kato; Masaaki Nakamura; Masaaki Ohba; Shinya Hayami

doi:10.1002/anie.202102774

Metal Complex Lipids for Fluid-Fluid Phase Separation in Coassembled Phospholipid Membranes

Angew Chem Int Ed Engl. 2021 Jun 7;60(24):13603-13608. doi: 10.1002/anie.202102774. Epub 2021 May 6.

Authors

Affiliations

¹ Department of Chemistry, Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
² Department of Chemistry, Graduate School of Science, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto, 860-8555, Japan.
³ Institute for Chemical Research, Kyoto University, Uji, Kyoto, 611-0011, Japan.
⁴ Exploratory Research Center on Life and Living Systems (ExCELLS) and Institute for Molecular Science (IMS), National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, 444-8787, Japan.
⁵ Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi, 467-8603, Japan.

PMID: 33723910
DOI: 10.1002/anie.202102774

Abstract

We demonstrate a fluid-fluid phase separation in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1-(2-hydroxybenzamide)-2-(2-hydroxy-3-formyl-5-hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids (T_c ) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant-unilamellar vesicles of DMPC/1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the T_c of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.

Keywords: amphiphile; lipid bilayer; metal-complex lipid; phase separation; supramolecules.

Publication types

Research Support, Non-U.S. Gov't