Long non-coding RNA MALAT1 regulates cell proliferation and apoptosis via miR-135b-5p/GPNMB axis in Parkinson's disease cell model

Biol Res. 2021 Mar 16;54(1):10. doi: 10.1186/s40659-021-00332-8.

Abstract

Backgrounds: Parkinson's disease (PD) is a common age-related neurodegenerative disorder worldwide. This research aimed to investigate the effects and mechanism underlying long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in PD.

Methods: SK-N-SH and SK-N-BE cells were treated with MPP+ to establish the MPP+-stimulated cell model of PD, and MALAT1 expression was determined. Then, the effects of MALAT1 depletion on cell proliferation and apoptosis were determined in the MPP+-stimulated cell model of PD. Besides, the correlations between microRNA-135b-5p (miR-135b-5p) and MALAT1 or glycoprotein nonmetastatic melanoma protein B (GPNMB) in MPP+-stimulated cell model of PD were explored.

Results: MALAT1 was increasingly expressed and downregulation of MALAT1 promoted cell proliferation while inhibited apoptosis in MPP+-stimulated cells. Besides, miR-135b-5p was a target of MALAT1 and directly targeted to GPNMB. Further investigation indicated that suppression of MALAT1 regulated cell proliferation and apoptosis by miR-135b-5p/GPNMB axis.

Conclusion: Our findings reveal that MALAT1/miR-135b-5p/GPNMB axis regulated cell proliferation and apoptosis in MPP+-stimulated cell model of PD, providing a potential biomarker and therapeutic target for PD.

Keywords: Apoptosis; Cell proliferation; GPNMB; MALAT1; Parkinson’s disease; miR-135b-5p.

MeSH terms

  • Apoptosis*
  • Cell Proliferation*
  • Cells, Cultured
  • Humans
  • Membrane Glycoproteins / genetics*
  • MicroRNAs / genetics*
  • Parkinson Disease / genetics*
  • RNA, Long Noncoding / genetics*

Substances

  • GPNMB protein, human
  • MALAT1 long non-coding RNA, human
  • MIRN135 microRNA, human
  • Membrane Glycoproteins
  • MicroRNAs
  • RNA, Long Noncoding