Bacillus amyloliquefaciens B10 can alleviate liver apoptosis and oxidative stress induced by aflatoxin B1

Food Chem Toxicol. 2021 May;151:112124. doi: 10.1016/j.fct.2021.112124. Epub 2021 Mar 13.

Abstract

Aflatoxin B1 (AFB1) is a mycotoxin often found in food and livestock feed. It can affect human and animal health and is especially damaging to the liver. This study aims to evaluate whether Bacillus amyloliquefaciens (hereafter referred to as B. amyloliquefaciens) B10 can alleviate the toxic effects of AFB1 and, if so, what mechanism is responsible for its action. Specific pathogen-free (SPF) Kunming mice (5-6 weeks old) were divided into four groups (Control, AFB1, B10 strain, and AFB1 + B10 strain) and conducted continuously via gavage for 28 days. Oxidation indices (MDA, T-AOC, SOD, GSH-Px, and CAT) were then measured using their liver tissues and liver coefficient were calculated. Apoptosis was determined using the TUNEL method. Gene expression was determined for Bax, Bcl-2, BIP, CHOP, JNK, Caspase-12, Caspase-9, and Caspase-3, and protein expression was detected for Bax, Bcl-2, and Caspase-3. Our results showed that AFB1 induced the oxidative damage and apoptosis in the livers of mice. However, for mice given B. amyloliquefaciens B10, the biochemical indices, pathological changes, the expressions of genes and proteins related to oxidative stress and apoptosis were significantly reversed. The results indicate that B. amyloliquefaciens B10 antagonizes oxidative damage and apoptosis induced by AFB1 in the livers of mice. The results of this study are of significance for the future use of this strain to reduce the harm of AFB1 to human health and animal reproductive performance.

Keywords: Aflatoxin B1; Apoptosis; Bacillus amyloliquefaciens B10; Liver; Oxidative stress.

MeSH terms

  • Aflatoxin B1 / toxicity*
  • Animals
  • Apoptosis / physiology*
  • Bacillus amyloliquefaciens / growth & development
  • Bacillus amyloliquefaciens / physiology*
  • Body Weight / drug effects
  • Liver / drug effects*
  • Liver / pathology
  • Mice
  • Oxidative Stress / drug effects*
  • Signal Transduction / drug effects*

Substances

  • Aflatoxin B1