Evaluation of paternal lymphocyte immunotherapy and potential biomarker mixed lymphocyte reaction-blocking factor in an Argentinian cohort of women with unexplained recurrent spontaneous abortion and unexplained infertility

Am J Reprod Immunol. 2021 Aug;86(2):e13422. doi: 10.1111/aji.13422. Epub 2021 Jun 3.

Abstract

Problem: Analyze the effect of paternal immunotherapy treatment (PIT) in primary and secondary unexplained recurrent spontaneous abortion (URSA) and unexplained infertility (UI).

Methods of study: A retrospective study analyzed a two-year follow-up between the generation of MLR-Bfs after PIT treatment (or controls first consultation) and a live birth. Recruited patients included primary URSA with two or more miscarriages at <12 weeks gestation, secondary URSA with previous live birth before two or more miscarriages, and UI with inability to conceive after 2 years of regular unprotected intercourse or in vitro fertilizations (IVF). PIT treated were compared with untreated controls.

Results: Primary URSA: live birth was 241/416 (58%) versus 64/282 (23%) controls (p < .0001). Up to age 35, success was 158/217 (73%) and 37/144 (26%) controls (p < .0001). With 3 or more previous URSA, success was 90/135 (67%) versus 17/79 (22%) controls (p < .0001). Between ages 36 and 40, success was 69/147(47%) versus 22/98 (22%) controls (p < .0003), with 3 or more previous URSA live birth was 45/95 (47%) versus 6/46 (13%) controls (p < .0001). In UI, live birth was 99/298 (33%) versus 54/263 (21%) in controls (p < .0009) that increased under age 35 to 53/116 (46%) in treated versus 26/101 (26%) controls (p < .0056). In PIT treated, IVF success required a median of 1 (1.37 ± 0.67) versus a median of 3 IVF procedures (2.75 ± 0.84) in controls.

Conclusion: PIT is a successful treatment for primary and secondary URSA, and UI. PIT reduced the number of IVF required for achieving pregnancy.

Keywords: IVF failures; fertilization in vitro; paternal immunotherapy; primary URSA; reproductive outcome; secondary URSA; unexplained infertility.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abortion, Habitual* / blood
  • Abortion, Habitual* / therapy
  • Adult
  • Antigens, Neoplasm / blood*
  • Biomarkers / blood
  • Female
  • Follow-Up Studies
  • Humans
  • Immunotherapy*
  • Infertility, Female* / blood
  • Infertility, Female* / therapy
  • Live Birth*
  • Lymphocyte Culture Test, Mixed
  • Lymphocyte Transfusion*
  • Retrospective Studies

Substances

  • Antigens, Neoplasm
  • Biomarkers
  • blocking factor