Curcumol inhibits the viability and invasion of colorectal cancer cells via miR-30a-5p and Hippo signaling pathway

Oncol Lett. 2021 Apr;21(4):299. doi: 10.3892/ol.2021.12560. Epub 2021 Feb 17.


MicroRNA-30a-5p (miR-30a-5p), which functions as a tumor suppressor, has been reported to be downregulated in colorectal cancer (CRC) tissues and to be associated with cancer invasion. However, the detailed regulatory mechanism of curcumol in the malignant progression of CRC remains unknown. MTT, Transwell, scratch, western blotting and reverse transcription-quantitative PCR assays were performed to examine how curcumol inhibited CRC cell viability, invasion and migration, and to detect the role of miR-30a-5p and curcumol in the invasion and Hippo signaling pathways of CRC cells. The present study revealed that miR-30a-5p expression was downregulated in human CRC tissues and cells. The results demonstrated that miR-30a-5p downregulation was accompanied by the inactivation of the Hippo signaling pathway, which was demonstrated to promote CRC cell viability, invasion and migration. Curcumol treatment was identified to increase miR-30a-5p expression and to activate the Hippo signaling pathway, which in turn inhibited the invasion and migration of CRC cells. Overexpression of miR-30a-5p enhanced the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. Furthermore, downregulation of miR-30a-5p reversed the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. These findings identified novel signaling pathways associated with miR-30a-5p and revealed the effects of curcumol on miR-30a-5p expression. Therefore, curcumol may serve as a potential therapeutic strategy to delay CRC progression.

Keywords: Hippo; colorectal cancer; curcumol; invasion; microRNA-30a-5p; viability.

Grant support

The present study was supported by the National Natural Science Foundation of China (grant nos. 81760443 and 81760663), Guangxi Special Fund Project for Innovation-Driven Development (grant no. GuikeAA19254025), the Project of Guangxi Natural Science Foundation (grant no. 2017GXNSFDA198029), the Fourth Batch of Bagui Scholars' Special Funds for 2017 [grant no. (2017) 143], the Small Talent Highland Fund in Guangxi (grant no. 201707), the Scientific Research and Technology Development Program of Guilin (grant no. 20170109-38), and Basic Ability Improvement Project for Young and Middle-aged People of Guangxi Education Department (grant no. 2020KY12030).