Association of genetic polymorphisms in vitamin D receptor (ApaI, TaqI and FokI) with vitamin D and glycemic status in type 2 diabetes patients from Southern India

Drug Metab Pers Ther. 2021 Mar 18;36(3):183-187. doi: 10.1515/dmpt-2020-0178.

Abstract

Objectives: To evaluate the association of VDR polymorphisms (FokI, TaqI and ApaI) with vitamin D levels and glycemic status in type 2 diabetes patients from Southern India.

Methods: In this observational study, genotype frequencies and vitamin D levels of 200 cases (type 2 diabetes patients) were compared with 300 controls (unrelated anonymised stored samples of healthy volunteers) from south India. Serum 25 (OH) D levels were measured by immunoassay technique, glycated hemoglobin (HbA1c) was measured using HPLC and genotyping of VDR polymorphisms were carried out using Real time Polymerase Chain Reaction (RT PCR).

Results: About 69.2% of type 2 diabetes patients were found to have vitamin D deficiency. FokI polymorphism showed variations in serum 25 (OH) D levels, with AA and AG genotypes having significantly lower serum 25 (OH) D levels as compared to GG [13.24 (8.4) ng/ml, 15.02 (7.07) ng/ml and 20.67 (13.64) ng/ml respectively]. There was no difference in HbA1c levels with respect to the vitamin D levels and VDR polymorphisms.

Conclusions: AA and AG genotypes of FokI polymorphisms are associated with low serum 25 (OH) D levels. However there was no association between VDR polymorphisms and glycemic status in south Indian type 2 diabetes patients.

Keywords: VDR polymorphisms; glycemic status; type 2 diabetic mellitus; vitamin D deficiency; vitamin D receptor (VDR).

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Glucose
  • Case-Control Studies
  • Diabetes Mellitus, Type 2* / genetics
  • Genetic Predisposition to Disease
  • Humans
  • India
  • Polymorphism, Genetic / genetics
  • Receptors, Calcitriol* / genetics
  • Vitamin D

Substances

  • Blood Glucose
  • Receptors, Calcitriol
  • Vitamin D