Comparison of the effectiveness of Ankaferd Blood Stopper® and Emdogain in periodontal regeneration

Oral Dis. 2022 Oct;28(7):1947-1957. doi: 10.1111/odi.13852. Epub 2021 Apr 1.


Objectives: The present study was performed to compare the effectiveness of Ankaferd Blood Stopper® (ABS) with enamel matrix derivatives (EMD) for treating fenestration defects in rats.

Materials and methods: Forty-eight male Wistar rats were randomly divided into six groups (each n = 8). Fenestration defects were created in all rats, to which ABS, EMD, or saline (S) was then applied. The rats were grouped and sacrificed at one of two different time points, as follows: ABS-10-group, ABS-treatment/sacrifice on day 10; EMD-10-group, EMD-treatment/sacrifice on day 10; S-10-group, S-treatment/sacrifice on day 10; ABS-38-group, ABS-treatment/sacrifice on day 38; EMD-38-group, EMD-treatment/sacrifice on day 38; and S-38-group, S-treatment/sacrifice on day 38. Then, histomorphometric analysis including measurements of new bone area (NBA) and new bone ratio (NBR), and immunohistochemical analysis including the determination of osteopontin (OPN) and type-III-collagen (C-III) expression were performed.

Results: The NBA and NBR were significantly higher in the ABS-10-group and EMD-10-group compared to the S-10-group (p < .05), and in the EMD-38-group compared to the S-38-group (p < .05). The levels of C-III and OPN immunoreactivity were significantly higher in the ABS-10-group compared to the S-10-group (p < .017).

Conclusions: The results of this study suggested that ABS can promote early periodontal regeneration, although its efficacy seems to decrease over time.

Keywords: Ankaferd Blood Stopper; Emdogain; histomorphometric; osteopontin; type-III collagen.

MeSH terms

  • Animals
  • Dental Enamel Proteins* / pharmacology
  • Dental Enamel Proteins* / therapeutic use
  • Male
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Wistar


  • Dental Enamel Proteins
  • Plant Extracts
  • ankaferd blood stopper