Purification and characterization of a fucoidan from the brown algae Macrocystis pyrifera and the activity of enhancing salt-stress tolerance of wheat seedlings

Int J Biol Macromol. 2021 Jun 1:180:547-558. doi: 10.1016/j.ijbiomac.2021.03.039. Epub 2021 Mar 16.

Abstract

A fuciodan (Mw = 11.1 kDa) was obtained and purified from Macrocystis pyrifera (MPF). MPF was an acid heteropolysaccharide including fucose, mannose, xylose, galactose, rhamnose, glucuronic acid, and glucose in a molar ratio of 3.1:1.0:0.86:0.63:0.25:0.33:0.11. Sulfate content in MPF was 28.6%, and the molar ratio of fucose to sulfate (Fuc:SO42-) was 1.0:0.58. The structure of MPF was mainly consist of repeating →3)-β-L-Fucp (2SO3-)-(1→ and →4)-β-D-Xylp-(1→3)-β-L-Fucp(2SO3-)-(1→ and with α-L-Fucp-(1→ and →6)-α-D-Galp-(1→ in branches. Moreover, the effects of different MPF concentrations on plant salt tolerance were investigated. The results indicated that MPF could improve the salt tolerance of wheat seedlings. Among the five concentrations (0.05, 0.1, 0.5, 1, and 2 mg/ml), 0.5 and 1 mg/ml MPF were optimal for effective plant salt-resistance activity. These results suggested that MPF extracted from brown seaweed show potential as plant stimulators that may be used to improve salt resistance of plants.

Keywords: Fucoidan; Macrocystis pyrifera; Salt tolerance.

MeSH terms

  • Antioxidants / metabolism
  • Dose-Response Relationship, Drug
  • Fucose / analysis
  • Hydrogen Peroxide / metabolism
  • Lipid Peroxidation / drug effects
  • Macrocystis / chemistry*
  • Magnetic Resonance Spectroscopy
  • Microscopy, Electron, Scanning
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology
  • Salt Stress / drug effects
  • Salt Stress / physiology*
  • Salt Tolerance / drug effects
  • Salt Tolerance / physiology*
  • Seedlings / drug effects
  • Seedlings / metabolism
  • Seedlings / physiology*
  • Spectroscopy, Fourier Transform Infrared
  • Sulfates / analysis
  • Superoxide Dismutase / metabolism
  • Triticum / drug effects
  • Triticum / metabolism
  • Triticum / physiology*

Substances

  • Antioxidants
  • Polysaccharides
  • Sulfates
  • Fucose
  • fucoidan
  • Hydrogen Peroxide
  • Superoxide Dismutase