Pulp tissue reaction to a self-adhesive, resin-based direct pulp capping material containing surface pre-reacted glass-ionomer filler

Dent Mater. 2021 Jun;37(6):972-982. doi: 10.1016/j.dental.2021.02.014. Epub 2021 Mar 17.


Objective: This study aimed to evaluate the effect of direct pulp capping using an experimental self-adhesive resin for direct pulp capping (SRD) containing silica and surface pre-reacted glass-ionomer (S-PRG) filler on pulpal healing and to monitor the dentin bridge formation in rat pulp 2-4 weeks after operation.

Methods: Five types of SRDs (SRD-0: S-PRG fillers 0 wt%; SRD-1: S-PRG fillers 9.1 wt%; SRD-2: S-PRG fillers 18.4 wt%; SRD-3: S-PRG fillers 27.8 wt%; and SRD-6: S-PRG fillers 57.4 wt%) were prepared, and mineral trioxide aggregate (MTA) was used as control (n = 8). Direct pulp capping was performed on rats that were sacrificed for further evaluation 2 or 4 weeks after the operation. The pulp tissue disorganization (PTD), inflammatory cell infiltration (ICI), and reparative dentin formation were histopathologically evaluated; the data were statistically analyzed using the Kruskal-Wallis and the Mann-Whitney U tests.

Results: The histopathological evaluation of SRD-1-treated test animals 2 weeks post-operation revealed inferior PTD and ICI when compared with that of MTA. Even 4 weeks after the operation in SRD-1- and SRD-2-treated rats, the PTD and ICI were inferior when compared with those of MTA. The dental specimens of SRD-0 and MTA showed orthodentin formation, whereas SRD-treated test animals showed osteodentin formation at a position slightly deeper than the site of the pulpal exposure.

Significance: The reparative dentin formed by SRD-0 and MTA was genuine, whereas that formed by SRD-3 and SRD-6 was ossified and ectopic. SRD may have the potential to be utilized clinically as a direct pulp capping material.

Keywords: Direct pulp capping; Filler contents; Multi-ion release; Self-adhesive resin; Surface pre-reacted glass-ionomer filler.

MeSH terms

  • Animals
  • Dental Cements
  • Dental Pulp
  • Dental Pulp Capping*
  • Pulp Capping and Pulpectomy Agents*
  • Rats
  • Resin Cements
  • Silicon Dioxide


  • Dental Cements
  • Pulp Capping and Pulpectomy Agents
  • Resin Cements
  • Silicon Dioxide