Generation of TCRα-transduced T cells for adoptive transfer therapy of salmonellosis in mice

STAR Protoc. 2021 Mar 9;2(1):100368. doi: 10.1016/j.xpro.2021.100368. eCollection 2021 Mar 19.

Abstract

Adoptive transfer therapy has great potential to treat diseases such as cancer as well as autoimmune and infectious diseases. Identification of chain-centric T cell receptors (TCRs) with the dominant-active antigen-specific α-chains (TCRα) can significantly improve the efficacy of adoptive cell therapy while reducing time, labor, and costs of generation of TCR-modified antigen-specific T cells. This protocol describes how to generate salmonella-specific TCRα-modified mouse T cells by retroviral transduction and evaluate their functional activity in vivo in the mouse model of salmonellosis. For complete details on the use and execution of this protocol, please refer to Kalinina et al. (2020).

Keywords: Cell biology; Cell isolation; Flow cytometry/mass cytometry; Immunology; Model organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer / methods*
  • Animals
  • Cell Separation / methods
  • Disease Models, Animal
  • Flow Cytometry / methods
  • Genetic Therapy / methods
  • Immunotherapy, Adoptive / methods*
  • Mice
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Salmonella Infections / therapy*
  • T-Lymphocytes / immunology
  • Transduction, Genetic

Substances

  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta