Retrogradely transmitted α-synuclein is taken up by the endophilin-independent endocytosis in the C. elegans neural circuit

Abstract

α-Synuclein is a major component of Lewy bodies and Lewy neuritis which are hallmarks of Parkinson's disease, and is known to propagate from cell-to-cell in a prion-like manner. However, the exact mechanism of α-synuclein propagation in cells remains unclear. Despite the increasing number of studies and models of α-synuclein propagation, there is no direct evidence demonstrating whether the propagation is trans-synaptic or synaptic connection-independent, what the direction of propagation is, and what the regulators of α-synuclein propagation are. In this study, we generated a Caenorhabditis elegans model that can help monitoring the neuron-to-neuron propagation of α-synuclein using BiFC system. Using this model, we demonstrated that α-synuclein was propagated into neurons in both anterograde and retrograde manners, with retrograde propagation being dominant. Interestingly, we also found that endophilin, which is a protein required for classical clathrin-mediated endocytic machinery, was not involved in this retrograde propagation. Furthermore, we demonstrated that α-synuclein inhibits neuronal activity through voltage-gated calcium channels. Our findings suggest a possible mechanism for α-synuclein propagation via synapses through a novel uptake pathway.

Keywords: C. elegans; Endocytosis; Parkinson’s disease; Retrograde transmission; α-synuclein.