A new species of Nilotanypus Kieffer is described and illustrated based on male, pupa and larva. Nilotanypus yanomami sp. n. was collected at madicolous system in a mountainous region of northern Amazonia. Additionally, the identification key for males is updated and a distribution map for the Neotropical species is provided.
Keywords: Diptera, Non-biting midges, Tanypodinae, Amazon rainforest, neotropics, new species.