Comparative bursal cytokine gene expression and apoptosis in vaccinated chickens following virulent infectious bursal disease virus challenge

Virology. 2021 Jun;558:126-133. doi: 10.1016/j.virol.2021.03.004. Epub 2021 Mar 18.

Abstract

The bursal cytokine gene expression and apoptosis were compared in vaccinated chickens with either live or immune-complex infectious bursal disease virus (IBDV) vaccines with or without virulent IBDV challenge. The cytokine gene expressions were evaluated at 5 and 12 day-post-challenge (DPC). The apoptotic marker Caspase-3 was determined by IHC on collected bursae, thymus, spleen, and kidneys at 12 DPC. A significantly decreased bursal cytokine levels were observed in the all-vaccinated birds except for IL-6 in the classic IBD vaccines at 5DPC. A significant upregulation of the IL-2 was observed in the live IBD vaccinated birds. No significant differences in the bursa and thymus Caspase-3 positive cells. However, splenic and renal apoptosis was significantly higher in the live IBD vaccine groups. Results indicate that both vaccine types reduce the IBDV-induced bursal proinflammatory cytokines and apoptosis. However, classic IBD vaccines failed to clear the challenge virus or reduce splenic and renal apoptosis.

Keywords: Apoptosis; Caspase-3; Cytokines; IBDV; Immune-complex; Vaccine.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Apoptosis / immunology*
  • Birnaviridae Infections / immunology
  • Birnaviridae Infections / prevention & control*
  • Birnaviridae Infections / veterinary*
  • Chickens
  • Cytokines / genetics*
  • Cytokines / immunology
  • Gene Expression / genetics
  • Gene Expression / immunology*
  • Infectious bursal disease virus / immunology*
  • Infectious bursal disease virus / pathogenicity
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*

Substances

  • Cytokines
  • Viral Vaccines