Zinc Prevents Ethanol-Induced Oxidative Damage in Lingual Tissues of Rats

Biol Trace Elem Res. 2022 Feb;200(2):720-727. doi: 10.1007/s12011-021-02682-6. Epub 2021 Mar 26.


The current study was designed to investigate the effects of zinc sulfate on cell proliferation, metallothionein (MT) immunoreactivity and antioxidant system against acute ethanol-induced oxidative damage in tongue tissues of rats. Wistar albino male rats, 2.5 to 3.0 months, were divided into four groups: Group I (n = 8), intact control rats; group II (n = 8), control animals given only zinc sulfate (100 mg/kg/day, for 3 consecutive days); group III (n = 14), animals given 1 mL absolute ethanol; group IV (n = 11), animals given zinc sulfate and absolute ethanol at the same dose and time. Animals were sacrificed under anesthesia 2 h after ethanol administration or 4 h after the last zinc sulfate treatment. Ethanol administration caused a marked decrease in the number of MT immunopositive cells and the proliferating cells in the lingual epithelium. A statistically significant decline in reduced glutathione levels, catalase activity and superoxide dismutase activities was also observed, whereas a significant elevation of lipid peroxidation levels and lactate dehydrogenase activities was detected in the ethanol group. In contrast, these changes were reversed by administration of zinc sulfate to ethanol-treated rats. In conclusion, it shows that zinc sulfate has therapeutic effects on acute ethanol-induced oxidative damage in the tongue tissues of rats.

Keywords: Ethanol; Lingual tissue; Metallothionein; Oxidative stress; Rat; Zinc sulfate.

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Antioxidants / pharmacology
  • Catalase / metabolism
  • Ethanol* / toxicity
  • Glutathione
  • Lipid Peroxidation
  • Oxidative Stress
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Tongue / metabolism
  • Zinc* / pharmacology


  • Antioxidants
  • Ethanol
  • Catalase
  • Superoxide Dismutase
  • Glutathione
  • Zinc