Therapeutic modality of induced uterine leiomyoma with shock waves in rats: The uterine blood flow, circulating ovarian hormones and histopathological findings

Rehab Saber Mamoon; Amany Sayed Mawas; Salwa Mostafa El Badry; Amel Mohamed Youssef; Mohamed Gamal Ali; Mohamed Aly Aly; Amal Mahmoud Abo El-Maaty

doi:10.1016/j.repbio.2021.100501

Therapeutic modality of induced uterine leiomyoma with shock waves in rats: The uterine blood flow, circulating ovarian hormones and histopathological findings

Reprod Biol. 2021 Jun;21(2):100501. doi: 10.1016/j.repbio.2021.100501. Epub 2021 Mar 24.

Authors

Rehab Saber Mamoon¹, Amany Sayed Mawas², Salwa Mostafa El Badry³, Amel Mohamed Youssef³, Mohamed Gamal Ali⁴, Mohamed Aly Aly⁵, Amal Mahmoud Abo El-Maaty⁵

Affiliations

¹ Department of Physical Therapy for Women's Health, South Valley University, Qena, Egypt; Department of Physical Therapy for Women's Health, Cairo University, Giza, Egypt. Electronic address: rehabsaber35@yahoo.com.
² Department of Pathology and Clinical Pathology, South Valley University, Qena, Egypt.
³ Department of Physical Therapy for Women's Health, Cairo University, Giza, Egypt.
⁴ Department of Physical Therapy for Women's Health, South Valley University, Qena, Egypt; Department of Physical Therapy for Women's Health, Cairo University, Giza, Egypt.
⁵ Department of Animal Reproduction and Artificial Insemination, National Research Centre, Giza, Egypt.

PMID: 33773235
DOI: 10.1016/j.repbio.2021.100501

Abstract

Uterine leiomyoma is the most common benign pelvic tumor and the primary indication for hysterectomy. We hypothesized tumor softening and shrinking through shock waves mechanobiological influence on fibroblasts of the induced leiomyoma in rats. Three rats served as control from thirty-three female Wistar rats subjected to leiomyoma induction using mono-sodium glutamate and estradiol benzoate. After assessing uterine leiomyoma development with Doppler ultrasonography, blood and tissue samples were collected for hormonal and histopathological analysis. Of the fifteen rats treated with shock waves, five rats were sacrificed after receiving two sessions (2S), another five rats were sacrificed after receiving four sessions (4S), and the last five rats were sacrificed after two weeks recovery period (recovered 4S). From the fifteen non-treated leiomyoma group, five rats were sacrificed after Doppler ultrasound assessment (Leiomyoma), another five rats were sacrificed with the 4S group (Leiomyoma 1Wk recovery), and the last five rats were sacrificed with the recovered 4S group (Recovered leiomyoma). The collected blood samples, estradiol (E2), Estrogen receptor, progesterone (P4), and progesterone receptor (PGR), were assayed. Total cholesterol, protein, albumin, and globulin were measured. Uterine arteries' blood flow velocities, indices, and volume were obtained. Tissue samples were stained with smooth muscle actin (SMA), trichrome-three, and (hematoxylin and eosin). Rats developed leiomyoma had the highest (P = 0.0001) gross and sonographic uterine horns diameters, uterine weight, uterine coefficient, E2, and ER. Both trichrome-three and SMA staining confirmed the leiomyoma development and the response to shock waves treatment. In conclusion, low-intensity shock waves proved curative to the induced leiomyoma.

Keywords: Monosodium glutamate; Rats; Shock waves; Uterine blood flow; Uterine leiomyoma.

MeSH terms

Animals
Estradiol / analogs & derivatives
Estradiol / toxicity
Extracorporeal Shockwave Therapy*
Female
Leiomyoma / chemically induced*
Leiomyoma / diagnostic imaging
Leiomyoma / therapy*
Rats
Receptors, Estrogen / blood
Receptors, Progesterone / blood
Sodium Glutamate / toxicity
Ultrasonography, Doppler

Substances

Receptors, Estrogen
Receptors, Progesterone
estradiol 3-benzoate
Estradiol
Sodium Glutamate