C19-diterpenoid alkaloids from the rhizomes of Aconitum pendulum

Junjie Wang; Huayong Lou; Jinyu Li; Ying Liu; Hongping Han; Zaichang Yang; Weidong Pan; Zhi Chen

doi:10.1016/j.fitote.2021.104887

C₁₉-diterpenoid alkaloids from the rhizomes of Aconitum pendulum

Fitoterapia. 2021 Jun:151:104887. doi: 10.1016/j.fitote.2021.104887. Epub 2021 Mar 26.

Authors

Junjie Wang¹, Huayong Lou², Jinyu Li², Ying Liu³, Hongping Han⁴, Zaichang Yang⁵, Weidong Pan⁶, Zhi Chen⁷

Affiliations

¹ Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province, Qinghai Normal University, Xining 810008, China; Bijie Medical College, Bijie 551700, China.
² State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China.
³ Bijie Medical College, Bijie 551700, China.
⁴ Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province, Qinghai Normal University, Xining 810008, China.
⁵ College of Pharmacy, Guizhou University, Guiyang 550025, China. Electronic address: yangzaichang@126.com.
⁶ State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China. Electronic address: wdpan@163.com.
⁷ Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province, Qinghai Normal University, Xining 810008, China. Electronic address: czi58@163.com.

PMID: 33775817
DOI: 10.1016/j.fitote.2021.104887

Abstract

Six new C₁₉-aconitine-type diterpenoid alkaloids, pendulumines A-F (1-6), together with six known ones (7-12), were isolated from the rhizomes of Aconitum pendulum. Their structures were elucidated using extensive spectroscopic data analysis, including 1D and 2D NMR, MS, and single-crystal X-ray diffraction analysis. The isolates were also tested for their analgesic activity based on the thermal avoidance response of the roundworm Pirstionchus pacificus, and 9 showed significant biological activity with an EC₅₀ value of 0.08 ± 0.02 mg.mL^-1.

Keywords: Aconitum pendulum; Analgesic activity; C(19)-diterpenoid alkaloids.

MeSH terms

Aconitum / chemistry*
Alkaloids / isolation & purification
Alkaloids / pharmacology*
Animals
China
Diterpenes / isolation & purification
Diterpenes / pharmacology*
Molecular Structure
Nematoda / drug effects*
Phytochemicals / isolation & purification
Phytochemicals / pharmacology
Rhizome / chemistry

Substances

Alkaloids
Diterpenes
Phytochemicals