Scaling Up DNA Origami Lattice Assembly

Chemistry. 2021 Jun 10;27(33):8564-8571. doi: 10.1002/chem.202100784. Epub 2021 May 4.


The surface-assisted hierarchical assembly of DNA origami nanostructures is a promising route to fabricate regular nanoscale lattices. In this work, the scalability of this approach is explored and the formation of a homogeneous polycrystalline DNA origami lattice at the mica-electrolyte interface over a total surface area of 18.75 cm2 is demonstrated. The topological analysis of more than 50 individual AFM images recorded at random locations over the sample surface showed only minuscule and random variations in the quality and order of the assembled lattice. The analysis of more than 450 fluorescence microscopy images of a quantum dot-decorated DNA origami lattice further revealed a very homogeneous surface coverage over cm2 areas with only minor boundary effects at the substrate edges. At total DNA costs of € 0.12 per cm2 , this large-scale nanopatterning technique holds great promise for the fabrication of functional surfaces.

Keywords: DNA origami; lattice formation; molecular lithography; self-assembly; topological analysis.

MeSH terms

  • DNA
  • Microscopy, Atomic Force
  • Nanostructures*
  • Nanotechnology*
  • Nucleic Acid Conformation


  • DNA