History, evolution and classification of CRISPR-Cas associated systems

Nisheeth Agarwal; Radhika Gupta

doi:10.1016/bs.pmbts.2020.12.012

History, evolution and classification of CRISPR-Cas associated systems

Prog Mol Biol Transl Sci. 2021:179:11-76. doi: 10.1016/bs.pmbts.2020.12.012. Epub 2021 Feb 9.

Authors

Nisheeth Agarwal¹, Radhika Gupta²

Affiliations

¹ Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, Haryana, India. Electronic address: nisheeth@thsti.res.in.
² Daulat Ram College, University of Delhi, New Delhi, Delhi, India. Electronic address: radhikagupta@dr.du.ac.in.

PMID: 33785174
DOI: 10.1016/bs.pmbts.2020.12.012

Abstract

This chapter provides a detailed description of the history of CRISPR-Cas and its evolution into one of the most efficient genome-editing strategies. The chapter begins by providing information on early findings that were critical in deciphering the role of CRISPR-Cas associated systems in prokaryotes. It then describes how CRISPR-Cas had been evolved into an efficient genome-editing strategy. In the subsequent section, latest developments in the genome-editing approaches based on CRISPR-Cas are discussed. The chapter ends with the recent classification and possible evolution of CRISPR-Cas systems.

Keywords: CRISPR-Cas system; CRISRPR; Cas; PAM; gRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Gene Editing*
Humans