Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

J Genet Eng Biotechnol. 2021 Mar 31;19(1):50. doi: 10.1186/s43141-021-00145-y.


Background: This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products.

Results: The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. The production of cellulase and amylase was depending on pH and incubation temperatures. ASP2 strain demonstrated the high production rate of amylase (at pH 5 and 30 °C) and cellulase (at pH 6 and 30 °C) for 96 h of incubation.

Conclusion: The A. niger showed the ability to produce several extracellular enzymes and can be used in the valorization of different agroindustrial residues.

Keywords: Aspergillus niger; Date by-products; Enzymes; Extracellular.