Preparation and characterization of clove essential oil loaded nanoemulsion and pickering emulsion activated pullulan-gelatin based edible film

Yi Shen; Zhi-Jing Ni; Kiran Thakur; Jian-Guo Zhang; Fei Hu; Zhao-Jun Wei

doi:10.1016/j.ijbiomac.2021.03.133

Preparation and characterization of clove essential oil loaded nanoemulsion and pickering emulsion activated pullulan-gelatin based edible film

Int J Biol Macromol. 2021 Jun 30:181:528-539. doi: 10.1016/j.ijbiomac.2021.03.133. Epub 2021 Mar 29.

Authors

Yi Shen¹, Zhi-Jing Ni², Kiran Thakur³, Jian-Guo Zhang⁴, Fei Hu⁵, Zhao-Jun Wei⁶

Affiliations

¹ School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.
² School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, People's Republic of China. Electronic address: lovebear@vip.163.com.
³ School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China; School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, People's Republic of China. Electronic address: kumarikiran@hfut.edu.cn.
⁴ School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China; School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, People's Republic of China. Electronic address: zhangjianguo@hfut.edu.cn.
⁵ School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China; School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, People's Republic of China. Electronic address: hufei@hfut.edu.cn.
⁶ School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China; School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, People's Republic of China. Electronic address: zjwei@hfut.edu.cn.

PMID: 33794240
DOI: 10.1016/j.ijbiomac.2021.03.133

Abstract

The clove essential oil (CEO) loaded nano and pickering emulsions prepared with Tween 80 and whey protein isolate/inulin mixture, respectively were incorporated into pullulan-gelatin film base fluid at three levels (0.2%, 0.4%, and 0.6%). The droplet sizes of NE and PE loaded with CEO were 15.93 nm and 266.9 nm, respectively. The PDI of CEOs with stable NE and PE were 0.262 and 0.259, respectively. Our results showed the improved compatibility between pullulan-gelatin and essential oil-loaded nanocarriers. The active film composed of PE carrier had the structural characteristics of high density, low water content, and low permeability, thus exhibiting excellent mechanical properties, water barrier properties, and appreciable antioxidant activities. Compared with NE, it was found that the CEO-loaded PE showed slow-release profile in the film sample. The prepared active film containing PE possessed a great potential to be used as effective and natural alternatives for active food packaging.

Keywords: Active packaging; Clove essential oil; Pickering emulsions; Pullulan-gelatin.

MeSH terms

Anti-Infective Agents / pharmacology
Antioxidants / pharmacology
Clove Oil / pharmacology*
Edible Films*
Emulsions / chemistry
Gelatin / chemistry*
Glucans / chemistry*
Microbial Sensitivity Tests
Microscopy, Atomic Force
Nanoparticles / chemistry*
Particle Size
Permeability
Spectroscopy, Fourier Transform Infrared
Steam
Temperature
Tensile Strength
Thermogravimetry
X-Ray Diffraction

Substances

Anti-Infective Agents
Antioxidants
Clove Oil
Emulsions
Glucans
Steam
gelatin film
pullulan
Gelatin