Deletion of Trpm4 Alters the Function of the Na v 1.5 Channel in Murine Cardiac Myocytes

Int J Mol Sci. 2021 Mar 26;22(7):3401. doi: 10.3390/ijms22073401.

Abstract

Transient receptor potential melastatin member 4 (TRPM4) encodes a Ca2+-activated, non-selective cation channel that is functionally expressed in several tissues, including the heart. Pathogenic mutants in TRPM4 have been reported in patients with inherited cardiac diseases, including conduction blockage and Brugada syndrome. Heterologous expression of mutant channels in cell lines indicates that these mutations can lead to an increase or decrease in TRPM4 expression and function at the cell surface. While the expression and clinical variant studies further stress the importance of TRPM4 in cardiac function, the cardiac electrophysiological phenotypes in Trpm4 knockdown mouse models remain incompletely characterized. To study the functional consequences of Trpm4 deletion on cardiac electrical activity in mice, we performed perforated-patch clamp and immunoblotting studies on isolated atrial and ventricular cardiac myocytes and surfaces, as well as on pseudo- and intracardiac ECGs, either in vivo or in Langendorff-perfused explanted mouse hearts. We observed that TRPM4 is expressed in atrial and ventricular cardiac myocytes and that deletion of Trpm4 unexpectedly reduces the peak Na+ currents in myocytes. Hearts from Trpm4-/- mice presented increased sensitivity towards mexiletine, a Na+ channel blocker, and slower intraventricular conduction, consistent with the reduction of the peak Na+ current observed in the isolated cardiac myocytes. This study suggests that TRPM4 expression impacts the Na+ current in murine cardiac myocytes and points towards a novel function of TRPM4 regulating the Nav1.5 function in murine cardiac myocytes.

Keywords: SCN5A; TRPM4; cardiac conduction disorder; channelosome; intracardiac ECG; mexiletine.

MeSH terms

  • Action Potentials
  • Animals
  • Arrhythmias, Cardiac / genetics*
  • Cells, Cultured
  • Gene Deletion
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Myocytes, Cardiac / metabolism*
  • Myocytes, Cardiac / physiology
  • NAV1.5 Voltage-Gated Sodium Channel / metabolism*
  • TRPM Cation Channels / genetics
  • TRPM Cation Channels / metabolism*
  • Ventricular Function

Substances

  • NAV1.5 Voltage-Gated Sodium Channel
  • Scn5a protein, mouse
  • TRPM Cation Channels
  • TRPM4 protein, mouse