Epidemiology and in vitro antimicrobial susceptibility of aerobic Actinomycetales in a clinical setting

Enferm Infecc Microbiol Clin. 2021 Mar 31;S0213-005X(21)00075-6. doi: 10.1016/j.eimc.2021.02.010. Online ahead of print.
[Article in English, Spanish]

Abstract

Introduction: The incidence of infections caused by aerobic actinomycetes is increasing. Recent changes in taxonomy and the variability in susceptibility patterns among species make necessary a proper identification and antibiotic susceptibility testing.

Material and methods: Fifty-three strains of aerobic actinomycetes were identified by MALDI-TOF MS using the VITEK MS Mycobacterium/Nocardia kit (bioMérieux, France) in a tertiary hospital in Spain during a six-year period. Antimicrobial susceptibility testing of the isolates was performed using the Sensititre Rapmycoi microdilution panel (Thermo Fisher Scientific, Massachusetts, USA).

Results: Forty strains of Nocardia spp. were identified in the study, being N. farcinica and N. cyriacigeorgica the most prevalent ones. All isolates were susceptible to linezolid and the resistance to amikacin was only observed in one isolate of Gordonia sputi. Resistance to cotrimoxazole was only found in five isolates.

Conclusions: Routine identification and antimicrobial susceptibility testing of aerobic actinomycetes is advisable for an efficient identification of species and effective treatment.

Keywords: Actinomycetales; Actinomycetes; Antimicrobial susceptibility; Gordonia; MALDI-TOF; Nocardia; Sensibilidad antimicrobiana; Tsukamurella.