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. 2021 Apr 5;253(5):91.
doi: 10.1007/s00425-021-03617-0.

Identification of two UDP-glycosyltransferases involved in the main oleanane-type ginsenosides in Panax japonicus var. major

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Identification of two UDP-glycosyltransferases involved in the main oleanane-type ginsenosides in Panax japonicus var. major

Jun-Rong Tang et al. Planta. .

Abstract

Two UDP-glycosyltransferases from Panax japonicus var. major were identified, and the biosynthetic pathways of three oleanane-type ginsenosides (chikusetsusaponin IVa, ginsenoside Ro, zingibroside R1) were elucidated. Chikusetsusaponin IVa and ginsenoside Ro are primary active components formed by stepwise glycosylation of oleanolic acid in five medicinal plants of the genus Panax. However, the key UDP-glycosyltransferases (UGTs) in the biosynthetic pathway of chikusetsusaponin IVa and ginsenoside Ro are still unclear. In this study, two UGTs (PjmUGT1 and PjmUGT2) from Panax japonicus var. major involved in the biosynthesis of chikusetsusaponin IVa and ginsenoside Ro were identified based on bioinformatics analysis, heterologous expression and enzyme assays. The results show that PjmUGT1 can transfer a glucose moiety to the C-28 carboxyl groups of oleanolic acid 3-O-β-D-glucuronide and zingibroside R1 to form chikusetsusaponin IVa and ginsenoside Ro, respectively. Meanwhile, PjmUGT2 can transfer a glucose moiety to oleanolic acid 3-O-β-D-glucuronide and chikusetsusaponin IVa to form zingibroside R1 and ginsenoside Ro. This work uncovered the biosynthetic mechanism of chikusetsusaponin IVa and ginsenoside Ro, providing the rational production of valuable saponins through synthetic biology strategy.

Keywords: Biosynthetic pathway; Heterologous expression; Oleanane-type ginsenosides; Saponins; Secondary metabolism; Uridine diphosphate glycosyltransferase.

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References

    1. Augustin JM, Kuzina V, Andersen SB, Bak S (2011) Molecular activities, biosynthesis and evolution of triterpenoid saponins. Phytochemistry 72(6):435–457 - PubMed - DOI - PMC
    1. Augustin JM, Drok S, Shinoda T, Biotech S (2012) UDP-glycosyltransferases from the UGT73C subfamily in Barbarea vulgaris catalyze sapogenin 3-O-glucosylation in saponin-mediated insect resistance. Plant Physiol 160(4):1881–1895 - PubMed - PMC - DOI
    1. Ayeleso TB, Matumba MG, Mukwevho E (2017) Oleanolic acid and its derivatives: biological activities and therapeutic potential in chronic diseases. Molecules 22(11):1915 - PMC - DOI
    1. Barvkar VT, Pardeshi VC, Kale SM, Kadoo NY, Gupta VS (2012) Phylogenomic analysis of UDP glycosyltransferase 1 multigene family in Linum usitatissimum identified genes with varied expression patterns. BMC Genomics 13(1):175 - PubMed - PMC - DOI
    1. Bönisch F, Frotscher J, Stanitzek S, Rühl E, Wüst M, Bitz O, Schwab W (2014) A UDP-glucose: monoterpenol glucosyltransferase adds to the chemical diversity of the grapevine metabolome (Vitis vinifera L). Plant Physiol 165(2):561–581 - PubMed - PMC - DOI

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