Immune-Enhancing Effects of Lactobacillus plantarum 200655 Isolated from Korean Kimchi in a Cyclophosphamide-Induced Immunocompromised Mouse Model

J Microbiol Biotechnol. 2021 May 28;31(5):726-732. doi: 10.4014/jmb.2103.03028.


In this study, we evaluated the immune-enhancing activity of kimchi-derived Lactobacillus plantarum 200655 on immune suppression by cyclophosphamide (CP) in ICR mice. Animals were fed distilled water or 1×109 colony-forming unit/kg B.W. 200655 or Lactobacillus rhamnosus GG as a positive control for 14 days. An in vivo model of immunosuppression was induced using CP 150 and 100 mg/kg B.W. at 7 and 10 days, respectively. Body weight, spleen index, spleen weight, and gene expression were measured to estimate the immune-enhancing effects. The dead 200655 (D-200655) group showed an increased spleen weight compared to the sham control (SC) group. Similarly, the spleen index was significantly higher than that in the CP-treated group. The live 200655 (L-200655) group showed an increased mRNA expression of tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 in splenocytes. Also, the iNOS and COX-2 mRNA expression was upregulated in the L-200655 group compared to the CP-only (SC) group. The phosphorylation of ERK and MAPK was also upmodulated in the L-200655 group. These results indicate that L. plantarum 200655 ameliorated CP-induced immune suppression, suggesting that L. plantarum 200655 may have the potential to enhance the immune system.

Keywords: Cyclophosphamide; Lactobacillus plantarum; MAPK/ERK signaling pathway; immune enhancement; splenocytes.

MeSH terms

  • Alanine Transaminase / blood
  • Animals
  • Cyclophosphamide / pharmacology
  • Cytokines / metabolism
  • Disease Models, Animal
  • Fermented Foods / microbiology*
  • Immunocompromised Host / drug effects*
  • Immunocompromised Host / immunology
  • Killer Cells, Natural / drug effects
  • Killer Cells, Natural / immunology
  • Lactobacillus plantarum* / isolation & purification
  • MAP Kinase Signaling System / drug effects
  • Mice
  • Mice, Inbred ICR
  • Probiotics / administration & dosage
  • Probiotics / pharmacology*
  • Republic of Korea
  • Spleen / drug effects
  • Spleen / immunology


  • Cytokines
  • Cyclophosphamide
  • Alanine Transaminase