Sensitive protein alignments at tree-of-life scale using DIAMOND

Nat Methods. 2021 Apr;18(4):366-368. doi: 10.1038/s41592-021-01101-x. Epub 2021 Apr 7.


We are at the beginning of a genomic revolution in which all known species are planned to be sequenced. Accessing such data for comparative analyses is crucial in this new age of data-driven biology. Here, we introduce an improved version of DIAMOND that greatly exceeds previous search performances and harnesses supercomputing to perform tree-of-life scale protein alignments in hours, while matching the sensitivity of the gold standard BLASTP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Computational Biology / methods*
  • Proteins / chemistry*
  • Sequence Alignment*


  • Proteins

Associated data

  • figshare/10.6084/m9.figshare.c.5053112.v1
  • figshare/10.6084/m9.figshare.14071334.v1