Functional Role of Long-Chain Acyl-CoA Synthetases in Plant Development and Stress Responses

Abstract

Fatty acids (FAs) play vital roles in plants as components of lipid membranes that demarcate cells and organelles, as sources of stored energy in the form of neutral lipids, and as signaling molecules that elicit plant responses to adverse conditions. The activation of FAs through the formation of acyl-CoA intermediates by acyl-CoA synthetase (ACS) family enzymes is required for their synthesis and degradation. Long-chain ACSs (LACSs) represent a small subgroup of ACS enzymes that specifically convert long-chain or very-long-chain FAs into corresponding thioesters for multiple lipid-associated processes. Alteration of LACS activity often results in pleiotropic phenotypes such as male sterility, organ fusion, aberrant cuticular structure, delayed seed germination, altered seed oil content, and plant capacity to respond to various environmental stresses. This review provides a comprehensive analysis of LACS family enzymes including substrate specificity, tissue-specific expression patterns, and distinct subcellular localization highlighting their specific roles in lipid synthesis and degradation, the effects of altered LACS activity on plant development, the relationship between LACS activity and stress resistance, and the regulation of LACS activity. Finally, we pose several major questions to be addressed, which would advance our current understanding of LACS function in plants.

Keywords: biosynthesis; lipid; long-chain acyl-CoA synthetases; metabolism; regulation; stress.

FIGURE 1

Expression pattern of AtLACS genes in different organs or tissues. All genes are from Arabidopsis thaliana. Genes specifically expressed in certain organs or tissues are marked with red color.

FIGURE 2

The possible roles of long-chain acyl-CoA synthetases (LACSs) residing in different subcellular compartments. The proteins shown here are from Arabidopsis thaliana (At), Brassica napus (Bn), Gossypium hirsutum (Gh), Helianthus annuus (Ha), and Oryza sativa (Os). Abbreviations: FAS, fatty acid synthesis complex; FAE, fatty acid elongation complex; TAG, triacylglycerol; LCFA, long-chain fatty acid; VLCFA, very-long-chain fatty acid.