A Novel Graphene Quantum Dot-Based mRNA Delivery Platform

ChemistryOpen. 2021 Jul;10(7):666-671. doi: 10.1002/open.202000200. Epub 2021 Apr 7.

Abstract

During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh-7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first-generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.

Keywords: drug delivery; functionalization; graphene quantum dots; hepatocarcinoma; mRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Membrane Permeability
  • Cell Survival / drug effects
  • Fluorescent Dyes / chemistry*
  • Graphite / chemistry*
  • Humans
  • Optical Imaging
  • Polyethyleneimine / chemistry*
  • Quantum Dots / chemistry*
  • Quantum Dots / metabolism
  • RNA, Messenger / chemistry*
  • RNA, Messenger / metabolism
  • Transfection

Substances

  • Fluorescent Dyes
  • RNA, Messenger
  • Graphite
  • Polyethyleneimine