Generation of GPAM knockout human embryonic stem cell line SYSUe-008-A using CRISPR/Cas9

Stem Cell Res. 2021 May;53:102303. doi: 10.1016/j.scr.2021.102303. Epub 2021 Mar 19.

Abstract

GPAM (glycerol-3-phosphateacyltransferase1) is a mitochondrial enzyme that catalyze an essential step in glycerolphospholipids and triacylglycerol biosynthesis process. Loss-of-function mutation of GPAM has been shown to lead to hypomyelination of corticospinal tract in cerebral palsy patient. To model this rare disease with human brain organoid, we generated a GPAM knockout human embryonic stem cell line SYSUe-008-A by CRISPR/cas9. The GPAM knockout cell line maintains a normal karyotype and shows comparable level of pluripotent stem cell marker expression and differentiation potential as wild-type human embryonic stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems / genetics
  • Cell Line
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Embryonic Stem Cells
  • Human Embryonic Stem Cells*
  • Humans