Purification and properties of formate dehydrogenase from Moraxella sp. strain C-1

J Bacteriol. 1988 Jul;170(7):3189-93. doi: 10.1128/jb.170.7.3189-3193.1988.


NAD+-dependent formate dehydrogenase was screened in various bacterial strains. Facultative methanol-utilizing bacteria isolated from soil samples, acclimated to a medium containing methanol and formate at pH 9.5, were classified as members of the genus Moraxella. From a crude extract of Moraxella sp. strain C-1, formate dehydrogenase was purified to homogeneity, as judged by disc gel electrophoresis. The enzyme has an isoelectric point of 3.9 and a molecular weight of approximately 98,000. The enzyme is composed of two identical subunits with molecular weights of about 48,000. The apparent Km values for sodium formate and NAD+ were calculated to be 13 mM and 0.068 mM, respectively.

MeSH terms

  • Aldehyde Oxidoreductases / isolation & purification*
  • Chromatography, High Pressure Liquid
  • Culture Media
  • Electrophoresis, Polyacrylamide Gel
  • Formate Dehydrogenases / analysis
  • Formate Dehydrogenases / isolation & purification*
  • Formates / metabolism
  • Hydrogen-Ion Concentration
  • Isoelectric Point
  • Kinetics
  • Methanol / metabolism
  • Molecular Weight
  • Moraxella / enzymology*
  • Moraxella / growth & development
  • Soil Microbiology*
  • Substrate Specificity
  • Temperature


  • Culture Media
  • Formates
  • Formate Dehydrogenases
  • Aldehyde Oxidoreductases
  • Methanol