Rapid, simultaneous determination of isomers of retinal, retinal oxime and retinol by high-performance liquid chromatography

J Chromatogr. 1988 Apr 22;438(2):383-92. doi: 10.1016/s0021-9673(00)90269-3.


Thirteen geometric isomers of retinol, retinal and retinal oxime were resolved in a single chromatographic run on two 250 X 4 mm 5-micron LiChrosorb Si 60 columns in series, using 11.2% ethyl acetate, 1.4% 1-octanol and 2% dioxane in hexane as the mobile phase. All the 11-cis and all-trans isomers of retinol and retinal oxime were completely resolved from each other and from the 9-cis and 13-cis isomers. This chromatographic procedure should be particularly useful for quantifying geometric isomers of retinoids in methylene chloride-hydroxylamine extracts of biological samples such as ocular tissues.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Chromatography, High Pressure Liquid
  • Diterpenes
  • Isomerism
  • Lighting
  • Oximes / analysis*
  • Reference Standards
  • Retina / analysis
  • Retinaldehyde / analysis*
  • Retinoids / analysis*
  • Stereoisomerism
  • Vitamin A / analysis*


  • Diterpenes
  • Oximes
  • Retinoids
  • Vitamin A
  • retinaloxime
  • Retinaldehyde